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tlr ligand  (InvivoGen)


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    Structured Review

    InvivoGen tlr ligand
    Ex vivo induction of IFNγ production after stimulation by <t>TLR</t> ligand. PBMC isolated from female conventional and SPF baboons were stimulated with TLR <t>ligands</t> <t>TLR1,</t> <t>TLR5,</t> <t>TLR6,</t> <t>TLR4,</t> <t>TLR3</t> and TLR8 for 24 h, and cells were evaluated in IFNγ ELISpot assays. P values were obtained by using the Student t test; data are presented as mean ±SEM (n = 5). Differences were considered significant at ** P < 0.05, ***, P < 0.005, ****, P < 0.0002 .
    Tlr Ligand, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr+ligands+fsl+1/FSL-1/pmc07137550-116-24-43
    Average 97 stars, based on 623 article reviews
    tlr ligand - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Comparative Analysis of Cellular Immune Responses in Conventional and SPF Olive Baboons ( Papio anubis )"

    Article Title: Comparative Analysis of Cellular Immune Responses in Conventional and SPF Olive Baboons ( Papio anubis )

    Journal: Comparative Medicine

    doi: 10.30802/AALAS-CM-19-000035

    Ex vivo induction of IFNγ production after stimulation by TLR ligand. PBMC isolated from female conventional and SPF baboons were stimulated with TLR ligands TLR1, TLR5, TLR6, TLR4, TLR3 and TLR8 for 24 h, and cells were evaluated in IFNγ ELISpot assays. P values were obtained by using the Student t test; data are presented as mean ±SEM (n = 5). Differences were considered significant at ** P < 0.05, ***, P < 0.005, ****, P < 0.0002 .
    Figure Legend Snippet: Ex vivo induction of IFNγ production after stimulation by TLR ligand. PBMC isolated from female conventional and SPF baboons were stimulated with TLR ligands TLR1, TLR5, TLR6, TLR4, TLR3 and TLR8 for 24 h, and cells were evaluated in IFNγ ELISpot assays. P values were obtained by using the Student t test; data are presented as mean ±SEM (n = 5). Differences were considered significant at ** P < 0.05, ***, P < 0.005, ****, P < 0.0002 .

    Techniques Used: Ex Vivo, Isolation, Enzyme-linked Immunospot

    Related Articles

    Transfection:

    Article Title: Evaluation of TLR Agonists as Potential Mucosal Adjuvants for HIV gp140 and Tetanus Toxoid in Mice
    Article Snippet: The TLR ligands FSL-1 (TLR2/6), Poly I∶C (TLR3), Pam 3 CSK 4 (TLR1/2), R848 (TLR7/8) were purchased from Invivogen, monophosphoryl Lipid A (MPLA, TLR4) from SIGMA and CpGB (TLR9) from MWG.

    Luciferase:

    Article Title: Evaluation of TLR Agonists as Potential Mucosal Adjuvants for HIV gp140 and Tetanus Toxoid in Mice
    Article Snippet: The TLR ligands FSL-1 (TLR2/6), Poly I∶C (TLR3), Pam 3 CSK 4 (TLR1/2), R848 (TLR7/8) were purchased from Invivogen, monophosphoryl Lipid A (MPLA, TLR4) from SIGMA and CpGB (TLR9) from MWG.

    Control:

    Article Title: Evaluation of TLR Agonists as Potential Mucosal Adjuvants for HIV gp140 and Tetanus Toxoid in Mice
    Article Snippet: The TLR ligands FSL-1 (TLR2/6), Poly I∶C (TLR3), Pam 3 CSK 4 (TLR1/2), R848 (TLR7/8) were purchased from Invivogen, monophosphoryl Lipid A (MPLA, TLR4) from SIGMA and CpGB (TLR9) from MWG.

    Plasmid Preparation:

    Article Title: Evaluation of TLR Agonists as Potential Mucosal Adjuvants for HIV gp140 and Tetanus Toxoid in Mice
    Article Snippet: The TLR ligands FSL-1 (TLR2/6), Poly I∶C (TLR3), Pam 3 CSK 4 (TLR1/2), R848 (TLR7/8) were purchased from Invivogen, monophosphoryl Lipid A (MPLA, TLR4) from SIGMA and CpGB (TLR9) from MWG.

    Activity Assay:

    Article Title: Evaluation of TLR Agonists as Potential Mucosal Adjuvants for HIV gp140 and Tetanus Toxoid in Mice
    Article Snippet: The TLR ligands FSL-1 (TLR2/6), Poly I∶C (TLR3), Pam 3 CSK 4 (TLR1/2), R848 (TLR7/8) were purchased from Invivogen, monophosphoryl Lipid A (MPLA, TLR4) from SIGMA and CpGB (TLR9) from MWG.



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    Image Search Results


    Ex vivo induction of IFNγ production after stimulation by TLR ligand. PBMC isolated from female conventional and SPF baboons were stimulated with TLR ligands TLR1, TLR5, TLR6, TLR4, TLR3 and TLR8 for 24 h, and cells were evaluated in IFNγ ELISpot assays. P values were obtained by using the Student t test; data are presented as mean ±SEM (n = 5). Differences were considered significant at ** P < 0.05, ***, P < 0.005, ****, P < 0.0002 .

    Journal: Comparative Medicine

    Article Title: Comparative Analysis of Cellular Immune Responses in Conventional and SPF Olive Baboons ( Papio anubis )

    doi: 10.30802/AALAS-CM-19-000035

    Figure Lengend Snippet: Ex vivo induction of IFNγ production after stimulation by TLR ligand. PBMC isolated from female conventional and SPF baboons were stimulated with TLR ligands TLR1, TLR5, TLR6, TLR4, TLR3 and TLR8 for 24 h, and cells were evaluated in IFNγ ELISpot assays. P values were obtained by using the Student t test; data are presented as mean ±SEM (n = 5). Differences were considered significant at ** P < 0.05, ***, P < 0.005, ****, P < 0.0002 .

    Article Snippet: Aliquots of 1 × 10 5 cells were incubated at 37 °C in a 5% CO 2 atmosphere for 24 h with or without TLR ligand (1 μg/mL; TLR1 Pam3CSK4, TLR3 Poly I:C, TLR4 LPS, TLR5 Flagellin, TLR6 FSL1, RLR8 ssRNA40/LyoVec; all from Invivogen [San Diego, CA]) in a 96-well plate for IFNγ ELISpot assay; the ELISpot plate was developed as described earlier.

    Techniques: Ex Vivo, Isolation, Enzyme-linked Immunospot

    Figure 2. Effect of TLR ligands and cytokines on monocyte PD-1H expression. a) Isolated monocytes were treated with the indicated TLR ligands [TLR1/2: PAM3CSK4 (1 mg/ml), TLR2: HKLM (108 cells/ml), TLR3: Poly I:C (2 mg/ml), TLR4: LPS (200 ng/ml), TLR5: flagellin (1 mg/ml), TLR6/2: FSL1 (0.5 mg/ml), TLR7: Imiquimod (2 mg/ml), TLR8: ssRNA40 (2 mg/ml), TLR9: ODN2006 (5 mM)] or cytokines (c) and examined for PD-1H expression by flow cytometry. The bar graphs and dose response curves represent data (mean6SD) from 3 replicates. * indicates p,0.05 (One way ANOVA followed by Tukey test). b,d) Dose dependent effect of TLRs and cytokines was determined on five different donors. Mean +/- SD for 5 donors is shown. doi:10.1371/journal.pone.0109103.g002

    Journal: PloS one

    Article Title: Characterization of programmed death-1 homologue-1 (PD-1H) expression and function in normal and HIV infected individuals.

    doi: 10.1371/journal.pone.0109103

    Figure Lengend Snippet: Figure 2. Effect of TLR ligands and cytokines on monocyte PD-1H expression. a) Isolated monocytes were treated with the indicated TLR ligands [TLR1/2: PAM3CSK4 (1 mg/ml), TLR2: HKLM (108 cells/ml), TLR3: Poly I:C (2 mg/ml), TLR4: LPS (200 ng/ml), TLR5: flagellin (1 mg/ml), TLR6/2: FSL1 (0.5 mg/ml), TLR7: Imiquimod (2 mg/ml), TLR8: ssRNA40 (2 mg/ml), TLR9: ODN2006 (5 mM)] or cytokines (c) and examined for PD-1H expression by flow cytometry. The bar graphs and dose response curves represent data (mean6SD) from 3 replicates. * indicates p,0.05 (One way ANOVA followed by Tukey test). b,d) Dose dependent effect of TLRs and cytokines was determined on five different donors. Mean +/- SD for 5 donors is shown. doi:10.1371/journal.pone.0109103.g002

    Article Snippet: Freshly isolated monocytes were cultured with various TLR ligands (TLR1/2: PAM3CSK4, TLR2: HKLM, TLR3: Poly I:C, TLR4: LPS, TLR5: flagellin, TLR6/2: FSL1, TLR7: Imiquimod, TLR8: ssRNA40, TLR9: ODN2006; Invivogen human TLR agonist kit) and examined for PD-1H expression by flow cytometry.

    Techniques: Expressing, Isolation, Flow Cytometry